Typing of sandflies from Greece and Cyprus by DNA polymorphism of 18S rRNA gene

Publication Type:Journal Article
Year of Publication:1999
Authors:Aransay, A. M., Scoulica, E., Chaniotis, B., Tselentis, Y.
Journal:Insect Molecular BiologyInsect Molecular Biology
Date Published:1999///
Keywords:18S ribosomal DNA, Diptera, PCR-RFLP, Phlebotomine sandflies, Typing

A simple and reliable technique was developed to distinguish Phlebotomine sandflies by restriction fragment length polymorphism of PCR-amplified (PCR-RFLP) 18S rDNAs. Seven morphologically identified sandflies species from several localities of Greece and Cyprus were studied, and specific patterns were developed by double digesting amplified 18S rDNAs with HpaII and RsaI. Three additional species of the subgenus Larroussius were distinguished by a second double digestion with AccI and BanI. We have successfully applied the method on samples in which morphological characters were badly distinguished due to poor storage conditions and in larval stages.

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith