@article {10211, title = {Discrimination of the closely related biocontrol agents Macrolophus melanotoma (Hemiptera: Miridae) and M. pygmaeus using mitochondrial DNA analysis}, journal = {Bulletin of Entomological ResearchBulletin of Entomological Research}, volume = {93}, number = {6}, year = {2003}, note = {Cited By (since 1996): 1Export Date: 5 September 2006Source: ScopusReferences: Albajes, R., Alomar, O., Current and potential use of polyphagous predators (1999) Integrated Pest Disease Management in Greenhouse Crops, pp. 265-275. , Albajes, R., Lodovica Gullino, M., van Lenteren, J.C. \& Elad, Y. (Eds). 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A guide to their recognition (1994) Boletin de Sanidad Vegetal Plagas, 20, pp. 131-143; Hadrys, H., Balick, M., Scierwater, B., Applications of random amplified polymorphic DNA (RAPD) in molecular ecology (1992) Molecular Ecology, 1, pp. 55-63; Hillert, O., Ja?ckel, B., Plate, H.P., Macrolophus pygmaeus (Rambur 1839) (Heteroptera: Miridae) - Ein interressanter Nu?tzling im biologischen Pflanzenschutz (2002) Gesunde Pflanzen, 54, pp. 66-73; Holloway, G.J., Marriot, C.G., Crocker, H.J., Phenotypic plasticity in hoverflies: The relationship between colour pattern and season in Episyrphus balteatus and other Syrphidae (1997) Ecological Entomology, 22, pp. 425-432; Josifov, M., Zur taxonomie der pala?arktischen (1992) Macrolophus - Arten. Raichenbachia, 29, pp. 1-4; Knight, H.H., On the nature of colour patterns in Heteroptera with data on the effects produced by temperature and humidity (1924) Annals of the Entomological Society of America, 17, pp. 258-273; Kotaki, T., Effects of low temperature on diapause termination and body colour change in adults of a stink bug Plautia stali (1998) Physiological Entomology, 23, pp. 53-61; Lykouressis, D.P., Perdikis, D.C., Chalkia, C.A., The effects of natural enemies on aphid populations on processing tomato (1999) Entomologia Hellenica, 13, pp. 35-42; Lykouressis, D., Perdikis, D., Tsagarakis, A., Polyphagous mirids in Greece: Host plants and abundance in traps placed in some crops (2000) Bollettino del Laboratorio di Entomologia Agraria Filippo Silvestri, 56, pp. 57-68; Malausa, J.C., Lutte inte?gre?e sous serre: Les punaises pre?datrices Mirides dans les cultures de Solanace?es du sudest de la France (1989) P.H.M.-Revue Horticole, 298, pp. 39-43; Margaritopoulos, J.T., Mamuris, Z., Tsitsipis, J.A., Attempted discrimination of Myzus persicae (Sulzer) and Myzus nicotianae Blackman (Homoptera: Aphididae) by random amplified polymorphic DNA polymerase chain reaction technique (1998) Annals of the Entomological Society of America, 91, pp. 602-607; Margaritopoulos, J.T., Tsitsipis, J.A., Perdikis, D.C., Biological characteristics of the mirids Macrolophus costalis and Macrolophus pygmaeus preying on the tobacco form of Myzus persicae (Hemiptera: Aphididae) (2003) Bulletin of Entomological Research, 93, pp. 39-45; Obrycki, J.J., Kring, T.J., Predaceous Coccinellidae in biological control (1998) Annual Review of Entomology, 43, pp. 295-321; Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L., Grabowski, G., (1991) The Simple Tool{\textquoteright}s Guide to PCR, , Department of Zoology, University of Hawaii, Honolulu, Hawaii; Perdikis, D., Lykouressis, D., The effects of various diets, host plants and temperatures on the development and survival of Macrolophus pygmaeus Rambur (Hemiptera: Miridae) (2000) Biological Control, 17, pp. 55-60; Perdikis, D., Lykouressis, D., Description of the eggs and the nymphal instars of Macrolophus pygmaeus Rambur (Hemiptera: Miridae) (2001) Entomologia Hellenica, 14, pp. 32-40; Perdikis, D., Lykouressis, D., Life table and biological characteristics of Macrolophus pygmaeus when feeding on Myzus persicae and Trialeurodes vaporariorum (2002) Entomologia Experimental et Applicata, 102, pp. 261-272; Putshkov, V.G., Species of the genus Macrolophus Fieber, 1858 (Heteroptera, Miridae) of the Soviet Union fauna (1978) Dopovidi Akademia Nauk URSR, pp. 853-856. , ser. B, Nr. 9; Schlinger, E.I., Doutt, R.L., Systematics in relation to biological control (1965) Biological Control of Insect Pests and Weeds, pp. 247-280. , DeBach, P. (Ed.). London, Chapman and Hall; Stichel, W., (1962) Illustrierte Bestimmungstabellen der Wanzen. II Europa (Hemiptera-Heteroptera Europae), 1-4, 2173p. , Berlin - Hermsdorf; Zitoudi, K., Margaritopoulos, J.T., Mamuris, Z., Tsitsipis, J.A., Genetic variation in Myzus persicae (Homoptera: Aphididae) populations associated with host-plant and life cycle category (2001) Entomologia Experimental et Applicata, 99, pp. 303-311.}, month = {2003///}, pages = {507-514}, abstract = {The separation of the closely related predatory species Macrolophus melanotoma Costa (= M. caliginosus Wagner) and Macrolophus pygmaeus (Rambur) based exclusively on the different colour pattern of the first antennal segment (white central band in M. melanotoma and entirely black in M. pygmaeus) is rather precarious and their taxonomic status is still in doubt. In the present study their taxonomic status was evaluated by DNA confirmatory analysis and hybridization experiments between M. pygmaeus and a Macrolophus taxon, resembling M. melanotoma, with a first antennal segment entirely black or with a white central band collected from Dittrichia viscosa (L.) W. Greuter (Asteraceae) in southern Greece. Adult females from Dittrichia plants hybridized with males of M. pygmaeus and vice versa did not produce viable eggs. The Macrolophus species from Dittrichia irrespective of the first antennal segment coloration differed from M. pygmaeus in digestive patterns generated by AseI, XbaI, and MseI on 16S rRNA and in RAPD profiles produced by the primers OPA-18 and OPA-20. These results demonstrate that on Dittrichia plants there is a distinct dimorphic taxon, M. melanotoma, as it is the only species of the genus Macrolophus bearing a first antennal segment with a central white band. Given the limitation of the coloration pattern, the mtDNA genetic markers are the appropriate method for the identification of M. melanotoma and M. pygmaeus.}, keywords = {Homoptera}, author = {Perdikis, D.C. and Margaritopoulos, J.T. and Stamatis, C. and Mamuris, Z. and Lykouressis, D.P. and Tsitsipis, J.A. and Pekas, A.} } @article {9297, title = {Genetic variation of Marchalina hellenica (Hemiptera: Margarodidae) sampled from different hosts and localities in Greece}, journal = {Bulletin of Entomological ResearchBulletin of Entomological Research}, volume = {93}, number = {5}, year = {2003}, note = {Cited By (since 1996): 1Export Date: 5 September 2006Source: ScopusReferences: Bacandritsos, N., (1998) Control of Mite Diseases of Honeybees without Use of Pesticides, , PhD thesis, Agricultural University of Athens (in Greek); Bacandritsos, N., A scientific note on the first successful establishment of the monophlebine coccid Marchalina hellenica (Coccoidea, Margarodidae) on the fir tree (Abies cephalonica) (2002) Apidologie, 33, pp. 353-354; Black, W.C., PCR with arbitrary primers: Approach with care (1993) Insect Molecular Biology, 2, pp. 1-6; Black W.C. IV, DuTeau, N.M., Puterka, G.J., Nechols, J.R., Pettorini, J.M., Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids (1992) Bulletin of Entomological Research, 82, pp. 151-159; Chatfield, C., Collins, A.J., Introduction to multivariate analysis (1980) London, Chapman \& Hall; Erlinghagen, F., Portrait of an insect: Marchallina hellenica Genn. (Sternorryhncha: Coccina: Margarodidae), important producer of honeydew in Greece (2001) Apiacta, 36, pp. 131-137; Fimiani, P., Sollino, G., An exotic insect dangerous to the native plants of the island of Ischia (1994) Informatore Agrario, 50, pp. 65-68; Frankham, R., Inbreeding and extinction: A threshold effect (1995) Conservation Biology, 9, pp. 792-799; Frankham, R., Conservation genetics (1995) Annual Review of Genetics, 29, pp. 305-327; Hadrys, H., Balick, M., Scierwater, B., Applications of random amplified polymorphic DNA (RAPD) in molecular ecology (1992) Molecular Ecology, 1, pp. 55-63; Holsinger, K.E., Paul, O.L., Dirak, K.D., A Bayesian approach to inferring population structure from dominant markers (2002) Molecular Ecology, 11, pp. 1157-1164; Jones, J.S., Can genes choose habitats? (1980) Nature, 286, pp. 757-758; Kailidis, D.L., Monophlebus hellenicus (Marchalina hellenica) Gen. The melitogenic insect of the pine tree (1965) Dasika Chronica, 81 (82), pp. 1-15. , in Greek; Kailidis, D.S., (1991) Forest Entomology and Zoology. 4th Edn., , Thessaloniki, Christodoulidis Press. (in Greek); Lynch, M., Milligan, B.G., Analysis of population genetic structure with RAPD markers (1994) Molecular Ecology, 3, pp. 91-99; Nei, M., Genetic distance between populations (1972) American Naturalist, 106, pp. 283-1232; Nikolopoulos, C., (1964) Sur la Decouverte de l{\textquoteright}Inconnum Jusqu{\textquoteright}aujourd{\textquoteright}hui Individuale de l{\textquoteright}Espece Marchalina Hellenica (Gennadius) (Hemiptera, Margarodidae, Coclostomidiinae), pp. 3-15. , Ecole de Hautes Etudes Agronomique Athenes, Lab. Zool. Agr. Et Series, 1964, in Greek; Nikolopoulos, C., (1965) Morphology and Biology of the Species Marchalina Hellenica (Gennadius) (Hemiptera, Margarodidae, Coclostomidiinae), pp. 3-31. , Ecole de Hautes Etudes Agronomique Athenes, Lab. Zool. Agr. Et Series, 1964, in Greek; Pollini, A., (1998) Manuale di Entomologia Applicata, , Bologna, Edagricole-Edizioni Agricole; Santas, L.A., Insects producing honeydew exploited by bees in Greece (1983) Apidologie, 14, pp. 93-103; Sneath, P.H.A., Sokal, R.R., (1973) Numerical Taxonomy, , San Francisco, W.H. Freeman \& Co; Sureyya, M., Hovasse, R., (1930) Les Ennemis des Pins Aux Iles Princes, , Istanbul; Spiegelhater, D.J., Best, N.G., Carlin, B.P., Van Der Linde, A., Bayesian measures of model complexity and fit (2002) Journal of the Royal Statistical Society Series B, 64, pp. 483-689; Thrasyvoulou, A., Manikis, I., Some physicochemical and microscopic characteristics of Greek unifloral honeys (1995) Apidologie, 26, pp. 441-452; Tremblay, E., (1995) Entomologia Applicata, 2. , Napoli, Liguori Editore; Wallner, W.E., Factors affecting insect population dynamics: Differences between outbreak and non-outbreak species (1987) Annual Review of Entomology, 32, pp. 317-340; Waples, R., A multispecies approach to the analysis of gene flow in marine shore fishes (1987) Evolution, 41, pp. 385-400; Williams, J.G.K., Hanafey, M.K., Rafalski, J.A., Tingey, S.V., Genetic analysis using random amplified polymorphic DNA markers (1993) Methods in Enzymology, 218, pp. 704-740.}, month = {2003///}, pages = {447-453}, abstract = {Random amplified polymorphic DNA (RAPD) analysis was applied to 120 individuals of Marchalina hellenica (Gennadius) representing six populations collected in northern, central and southern mainland Greece. One population was sampled on one species of fir tree and the others on two species of pine trees. Four random decamer primers were used to evaluate genetic variation among the populations examined. The results revealed intra- and interpopulation polymorphism both related to host type and region of origin. Phylogenetic analysis based on genetic distances estimated by the RAPD frequencies revealed an important genetic differentiation in samples collected on fir trees in southern Greece and to a lesser extent in samples from pine trees in central and northern Greece. Furthermore, considerable subdivision and restricted gene flow among the populations examined were observed. The results are discussed in relation to the biology and geographical distribution of M. hellenica in Greece.}, keywords = {Homoptera}, author = {Margaritopoulos, J.T. and Bacandritsos, N. and Pekas, A.N. and Stamatis, C. and Mamuris, Z. and Tsitsipis, J.A.} } @proceedings {9296, title = {A study on the genetic variation of Marchalina hellenica (Hemiptera: Margarodidae) Gennadius in Greece. }, year = {2002}, month = {7-13.10.2002}, pages = {133}, address = {Thessaloniki}, keywords = {kape homoptera}, author = {Margaritopoulos, J.T. and Bacandritsos, N. and Pekas, A.E. and Stamatis, C. and Mamuris, Z. and Tsitsipis, J.A.} }