01741nas a2200205   4500008004100000245013300041210006900174260001200243300001200255490000700267520100100274653001401275100002601289700002101315700001601336700001701352700001601369700002001385856013001405       2003                        eng d00aGenetic variation of <i>Marchalina hellenica </i>(Hemiptera: Margarodidae) sampled from different hosts and localities in Greece0 aGenetic variation of iMarchalina hellenica iHemiptera Margarodid  c2003///  a447-4530 v933 aRandom amplified polymorphic DNA (RAPD) analysis was applied to 120 individuals of Marchalina hellenica (Gennadius) representing six populations collected in northern, central and southern mainland Greece. One population was sampled on one species of fir tree and the others on two species of pine trees. Four random decamer primers were used to evaluate genetic variation among the populations examined. The results revealed intra- and interpopulation polymorphism both related to host type and region of origin. Phylogenetic analysis based on genetic distances estimated by the RAPD frequencies revealed an important genetic differentiation in samples collected on fir trees in southern Greece and to a lesser extent in samples from pine trees in central and northern Greece. Furthermore, considerable subdivision and restricted gene flow among the populations examined were observed. The results are discussed in relation to the biology and geographical distribution of M. hellenica in Greece.10aHomoptera1 aMargaritopoulos, J.T.1 aBacandritsos, N.1 aPekas, A.N.1 aStamatis, C.1 aMamuris, Z.1 aTsitsipis, J.A.  uhttps://icgf.myspecies.info/content/genetic-variation-marchalina-hellenica-hemiptera-margarodidae-sampled-different-hosts-and