02516nas a2200349   4500008004500000020001400045245012500059210006900184300001000253490000700263520143100270653001601701653001401717653001101731653001901742653002201761653001101783653001401794653001601808653001901824653003201843653001001875653003101885653001601916653001501932653001601947100002201963700002101985700001402006700001502020856013102035       2011                        Engldsh    a0021-883900aPhylogenetic relationships of Greek <i>Apis mellifera </i>subspecies based on sequencing of mtDNA segments (COI and ND5)0 aPhylogenetic relationships of Greek iApis mellifera isubspecies   a42-500 v503 aHoney bee Apis mellifera L. populations from various areas of Albania, Bulgaria, Cyprus, Greece, Italy, Slovenia and Turkey, together with a genetically improved commercial strain exported all over the world were studied using sequencing analysis of two mitochondrial regions, the ND5 and the COI gene segments. The aim of this study was to examine the phylogenetic relationships among these honey bee populations, to investigate the existence of gene flow as a result of migratory beekeeping and commercial breeding, and to find out if these subspecies can be discriminated. Seven and eight different haplotypes were revealed for the COI and ND5 gene segments respectively, while the combined data set consisted of twelve different haplotypes. Among the two DNA segments studied, the highest genetic divergence values were observed in COI. In both genes the highest divergence value was among A. m. ligustica colonies and all others. All the phylogenetic trees constructed by Maximum Parsimony, Neighbour-Joining and Bayesian Inference analyses exhibited exactly the same topology for COI and ND5 separately, as well as for the concatenated data set; A. m. ligustica forms the most distinct clade. Our study presents the first comprehensive sequencing analysis of A. mellifera subspecies occurring in Greece and it is the first time that sequencing data from ND5 mtDNA gene segment have been obtained at the population level.10a4TH LINEAGE10aALIGNMENT10aApidae10aApis mellifera10agenetic diversity10aGreece10aHONEY-BEE10aHymenoptera10amicrosatellite10aMITOCHONDRIAL-DNA VARIATION10amtDNA10aphylogenetic relationships10apopulations10asequencing10avariability1 aMartimianakis, S.1 aKlossa-Kilia, E.1 aBouga, M.1 aKilias, G.  uhttps://icgf.myspecies.info/content/phylogenetic-relationships-greek-apis-mellifera-subspecies-based-sequencing-mtdna-segments02709nas a2200301   4500008004500000020001400045245011800059210006900177260000800246300001000254490000800264520175300272653001402025653001702039653001402056653002202070653002502092653001802117653002602135653001702161653001602178653002102194653001302215100001802228700001902246700001702265856012502282       2009                        Engldsh    a0018-066100aPhylogeography and mitochondrial DNA divergence in <i>Dolichopoda </i>cave crickets (Orthoptera, Rhahidophoridae)0 aPhylogeography and mitochondrial DNA divergence in iDolichopoda   cMay  a33-450 v1463 aDolichopoda cave crickets are found in caves in the northern Mediterranean region from the Pyrenees in the west to the Caucasus in the east. In this study we analysed the phylogeny within the genus Dolichopoda using parts of the mitochondrial cytochrome oxidase I and 16S ribosomal genes, and explored biogeographic patterns through a dispersal-vicariance analysis (DIVA). Phylogenetic analyses grouped the 15 species into the four geographically restricted main lineages corresponding to the Caucasus, Greece, the Pyrenees and Italy, respectively. The species occur largely in allopatry. The Caucasian and Greek species were basal in the phylogeny, as was the clade including the nine Italian species, which grouped into two major lineages, one mainly including species from western Italian coastal regions and islands, and the other including species with a predominantly inland distribution. Thus it seems likely that there have been two main immigrations into Italy followed by multiple consecutive speciation events. The DIVA analysis supported the assumption of an east-west migration route, and indicated that there have been four major dispersal events. Since the statistical support for the basal node connecting D. remyi and D. hussoni with the west Mediterranean species is low, alternative interpretations for the colonization of the Mediterranean, namely parallel colonization of the main areas of the current Dolichopoda distribution, i.e. the Caucasus, Greece, Italy, and the Pyrenees is also possible. Particular emphasis was put on the D. geniculata-laetitiae species complex. D. geniculata included several recently diverged lineages and constitutes a paraphyletic species complex, also embracing the closely related D. laetitiae.10aALIGNMENT10abiogeography10aevolution10aGENETIC-STRUCTURE10aGEOGRAPHIC-VARIATION10ahybridization10aphylogenetic analysis10apolymorphism10apopulations10aRHAPHIDOPHORIDAE10asequence1 aMartinsen, L.1 aVenanzetti, F.1 aBachmann, L.  uhttps://icgf.myspecies.info/content/phylogeography-and-mitochondrial-dna-divergence-dolichopoda-cave-crickets-orthoptera