03134nas a2200265   4500008004100000245015600041210006900197260001200266300000900278520227400287653001202561653001302573653000802586653001102594653001602605653001302621653001402634653001202648653001502660100001802675700001902693700001502712700001402727856012702741       2005                        eng d00aPhylogenetic relationships of the subfamily Pyrgulinae (Gastropoda: Caenogastropoda: Hydrobiidae) with emphasis on the genus <i>Dianella </i>Gude, 19130 aPhylogenetic relationships of the subfamily Pyrgulinae Gastropod  c2005///  a1-323 aThe phylogenetic position of the subfamily Pyrgulinae within the superfamily Rissooidea has been discussed very controversially. Different data sets not only led to different evolutionary scenarios but also to different systematic classifications of the taxon. The present study uses detailed anatomical data for two pyrgulinid taxa, the type species of the subfamily, Pyrgula annulata (Linnaeus, 1767), and the type species of the little known genus Dianella, D. thiesseana (Kobelt, 1878), as well as DNA sequencing data of three gene fragments from representatives of eight rissooidean families to A) infer the phylogenetic position of Pyrgulinae with emphasis on its relationships within the family Hydrobiidae, B) to study the degree of concordance between anatomy- and DNA-based phylogenies and C) to trace the evolution of anatomical characters along a multi-gene molecular phylogeny to find the anatomical characters that might be informative for future cladistic analyses. Both anatomical and molecular data sets indicate either a very close or even sister-group relationship of Pyrgulinae and Hydrobiinae. However, there are major conflicts between the two data sets on and above the family level. Notably, Hydrobiidae is not monophyletic in the anatomical analysis. The reconstruction of anatomical character evolution indicates that many of the characters on which the European hydrobioid taxonomy is primarily based upon are problematic. The inability to clearly separate some hydrobiids from other distinct families based on those characters might explain why until only a few years ago, "Hydrobiidae" was a collecting box for numerous rissooidean taxa (mostly species with shells small and lacking any characteristic features). The present study not only stresses the need for comprehensive molecular studies of rissooidean taxa, it also demonstrates that much of the problems surrounding anatomical analyses in rissooidean taxa are due to the lack of comprehensive data for many representatives. In order to aid future comparative anatomical studies and a better understanding of character evolution in the species-rich family Hydrobiidae, detailed anatomical descriptions for P. annulata and D. thiesseana are provided. Copyright © 2005 Magnolia Press.10aanatomy10aDianella10aDNA10aGreece10aHydrobiidae10aMollusca10aphylogeny10aPyrgula10aPyrgulinae1 aSzarowska, M.1 aFalniowski, A.1 aRiedel, F.1 aWilke, T.  uhttps://icgf.myspecies.info/content/phylogenetic-relationships-subfamily-pyrgulinae-gastropoda-caenogastropoda-hydrobiidae03155nas a2200373   4500008004100000245015600041210006900197260000800266300001000274490000800284520197300292653001502265653001902280653002402299653001502323653001602338653001502354653002302369653002402392653001002416653002702426653002102453653000802474653002702482653001502509653001802524653002202542653001302564653002302577100001902600700001402619700001502633856013302648       2005                        eng d00aWhen DNA puts ecological works back on the right track: genetic assessment and distribution patterns of mudsnail populations in the Evros Delta lagoons0 aWhen DNA puts ecological works back on the right track genetic a  cJAN  a19-350 v1623 aThe present study aims to assess the distribution patterns of mudsnail populations in the Evros Delta lagoons and to investigate the key environmental variables affecting them. As a correct species assignment is crucial for ecological studies, the data presented here are substantiated using DNA sequences from the mitochondrial genome to verify the systematic status of the mudsnail taxon in question. A comparative molecular analysis involving almost all brackish-water taxa of Hydrobiinae indicates that the populations from the Evros Delta belong to the Pontic species Ventrosia maritima, a taxon that was not reported before from the Mediterranean. Moreover, it appears that in previous studies, the mudsnail populations from the Evros Delta were misidentified. Principal Component Analyses using environmental variables defined station groups among which V maritima showed a significant variation in density. Mudsnail specimens were absent at stations with an immediate access to the sea (salinity 32 - 34 PSU, very fine to fine sand, organic matter 0.3 -1.4 %), they showed intermediate densities (up to 3,000 ind./m(2)) in innermost lagoonal parts (34-35 PSU, very fine sand, organic matter 1.0-1.4 %) and relatively high densities (up to 7,000 ind./m(2)) only in isolated parts (15-17 PSU, fine sand, organic matter 0.5-0.8 %). Contact with the sea, which is strongly correlated with seawater renewal rate and hydrodynamism, was found to be the most important factor affecting mudsnail distributions. The data presented, being the first detailed ecological information available for V. maritima, are compared with the available information for other mudsnail taxa. The present study not only lays the foundation for future comparative eco-genetic works of mudsnail taxa, it also stresses the absolute need for the routine usage of molecular markers in ecological studies involving frequently utilized but systematically poorly understood invertebrate species.10aAegean sea10acoastal lagoon10aCommunity structure10aGastropoda10aHydrobiidae10alife-cycle10amacrobenthic fauna10aMollusca Gastropoda10amtDNA10amud snails hydrobiidae10anorth aegean sea10apca10aPHYLOGENETIC INFERENCE10asalt-marsh10asediment type10aspatial variation10aventrosa10aventrosia maritima1 aKevrekidis, T.1 aWilke, T.1 aMogias, A.  uhttps://icgf.myspecies.info/content/when-dna-puts-ecological-works-back-right-track-genetic-assessment-and-distribution-patterns