@conference {16487, title = {PHYLOGENETIC RELATIONSHIPS AND PHYLOGEOGRAPHY OF ABLEPHARUS KITAIBELII(SAURIA: SCINCIDAE) SPECIES COMPLEX IN EASTERN MEDITERRANEAN}, booktitle = {12TH INTERNATIONAL CONGRESS ON THE ZOOGEOGRAPHY AND ECOLOGY OF GREECE AND ADJACENT REGIONS}, year = {2012}, month = {18 {\textendash} 22 June}, publisher = {HELLENIC ZOOLOGICAL SOCIETY}, organization = {HELLENIC ZOOLOGICAL SOCIETY}, address = {Athens}, keywords = {BIOGEOGRAPHICAL, eastern Mediterranean, evolution, phylogenetic relationships, phylogeography, Reptilia, Sauria, Scincidae, species, topology, ανατολική Μεσόγειος, βιογεωγραφία, είδη, εξέλιξη, ερπετά, τοπολογία, φυλογενετικές σχέσεις, φυλογεωγραφία}, author = {Skourtanioti, E. and Kapli, P. and Lymberakis, P. and Ilgaz, C. and Kumlutas, Y. and Avc{\i}, A. and Ahmadzadeh, F. and Poulakakis, N.} } @conference {16419, title = {MOLECULAR PHYLOGENY OF HELIX CINCTA (M{\"U}LLER, 1774) AND HELIX NUCULA (MOUSSON, 1854) IN THE EASTERN MEDITERRANEAN}, booktitle = {12TH INTERNATIONAL CONGRESS ON THE ZOOGEOGRAPHY AND ECOLOGY OF GREECE AND ADJACENT REGIONS}, year = {2012}, month = {18 {\textendash} 22 June}, publisher = {HELLENIC ZOOLOGICAL SOCIETY}, organization = {HELLENIC ZOOLOGICAL SOCIETY}, address = {Athens}, keywords = {Helix cincta, Helix nucula, Aegean, Aνατολική Μεσόγειος, eastern Mediterranean, Molecular Phylogeny , snail, Αιγαίο, μοριακή φυλογενία, σαλιγκάρι}, author = {Psonis, N. and Vardinoyannis, K. and Mylonas, M. and Poulakakis, N.} } @conference {16417, title = {MOLECULAR PHYLOGENY AND PHYLOGEOGRAPHY OF THE TWO CLOSELY RELATED SNAIL GENERA HELICIGONA \& CAMPYLAEA (GASTROPODA: PULMONATA) IN GREECE}, booktitle = {12TH INTERNATIONAL CONGRESS ON THE ZOOGEOGRAPHY AND ECOLOGY OF GREECE AND ADJACENT REGIONS}, year = {2012}, month = {18 {\textendash} 22 June}, publisher = {HELLENIC ZOOLOGICAL SOCIETY}, organization = {HELLENIC ZOOLOGICAL SOCIETY}, address = {Athens}, keywords = {Campylaea, Gastropoda, Greece, Helicigona, Molecular Phylogeny , phylogenetic analyses, Pulmonata, snails, species, γαστρόποδα, είδη, Ελλάδα, μοριακή φυλογενία, σαλιγκάρια, φυλογενετικές αναλύσεις}, author = {Psonis, N. and Vardinoyannis, K. and Mylonas, M. and Poulakakis, N.} } @article {13355, title = {Combining immunological and molecular data to assess phylogenetic relations of some Greek Podarcis species}, journal = {Comparative Biochemistry and Physiology, Part BComparative Biochemistry and Physiology, Part B}, volume = {147}, number = {1}, year = {2007}, pages = {1}, publisher = {Elsevier}, author = {Efstratios D. Valakos and Kourkouli, A. and Skopeliti, M. and Pafilis, P. and Poulakakis, N. and Voutsas, I. F. and Lymberakis, P. and Simou, C. and Voelter, W. and Tsitsilonis, O. E.} } @article {10614, title = {Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae)}, journal = {Molecular Ecology NotesMolecular Ecology Notes}, volume = {5}, number = {3}, year = {2005}, pages = {549}, publisher = {Blackwell Synergy}, keywords = {Reptilia}, author = {Poulakakis, N. and Goulielmos, G. and Antoniou, A. and Zouros, E. and Mylonas, M.} } @article {10615, title = {Molecular phylogeny and biogeography of the wall-lizard Podarcis erhardii (Squamata: Lacertidae)}, journal = {Molecular Phylogenetics and EvolutionMolecular Phylogenetics and Evolution}, volume = {28}, number = {1}, year = {2003}, note = {Cited By (since 1996): 13Export Date: 5 September 2006Source: ScopusReferences: Arnold, E.N., Relationships of the Palaearctic lizards assigned to the genera Lacerta, Algyroides and Psammodromus (Reptilia: Lacertidae) (1973) Bull. Br. Mus. Nat. 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Kl., Wien, 166 (3-4), pp. 123-164.}, month = {2003///}, pages = {38-46}, abstract = {Erhard{\textquoteright}s wall lizard, Podarcis erhardii (Sauria: Lacertidae), is highly diversified in Greece and especially in the southern Aegean region. Out of the 28 recognized subspecies, 27 are found in Greece from the North Sporades island-complex in the North Aegean (grossly south of the 39th parallel) to the island of Crete in the South. The species exhibits great morphological and ecological plasticity and inhabits many different habitats from rocky islets and sandy shores to mountaintops as high as 2000 m. By examining intraspecific variability at a segment of the mitochondrial gene cytochrome b we have found that that extant populations of P. erhardii are paraphyletic. Furthermore, we have found that subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, so that their status should be reconsidered. The DNA based biogeographical and phylogenetic history of Podarcis in Southern Greece is congruent with available paleogeographic data of the region, which supports the view that DNA sequences may be a useful tool for the study of palaeogeography. {\textcopyright} 2003 Elsevier Science (USA). All rights reserved.}, keywords = {Reptilia}, author = {Poulakakis, N. and Lymberakis, P. and Antoniou, A. and Chalkia, D. and Zouros, E. and Mylonas, M. and Efstratios D. 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Evol., 10, pp. 512-526; Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tool (1997) Nucleic Acids Res., 24, pp. 4876-4882; Whiting, A.S., Bauer, A.M., Sites Jr., J.W., Phylogenetic relationships and limb loss in sub-Saharan African scincene lizards (Squamata: Scincidae) (2003) Mol. Phylogenet. Evol., 29, pp. 582-598.}, month = {2005///}, pages = {245-256}, abstract = {Sequence data derived from two mitochondrial markers, 16S rRNA and cytochrome b genes, were used to infer the phylogenetic relationships of 38 populations of the snake-eyed skinks of the genus Ablepharus with emphasis on A. kitaibelii from Greece and Turkey. The partition-homogeneity tests indicated that the combined data set was homogeneous, and maximum-parsimony, maximum-likelihood, and Bayesian analyses produced topologically identical trees that revealed a well-resolved phylogeny. All species except A. kitaibelii form monophyletic units. The latter species appears paraphyletic with respect to A. budaki and A. chernovi with populations clustering into two distinct clades. A. chernovi and A. budaki, which have recently been raised to species status, were confirmed as genetically distinct forms. We used sequence divergence and paleogeographic history of the Aegean region to reconstruct a biogeographic evolutionary scenario for A. kitaibelii. {\textcopyright} 2004 Elsevier Inc. All rights reserved.}, keywords = {16S rRNA, Ablepharus kitaibelii, Cytochrome b, phylogeny, phylogeography, Reptilia, Scincidae, systematics}, author = {Poulakakis, N. and Lymberakis, P. and Tsigenopoulos, C.S. and Magoulas, A. and Mylonas, M.} } @article {10618, title = {Phylogeography of Balkan wall lizard (Podarcis taurica) and its relatives inferred from mitochondrial DNA sequences}, journal = {Molecular EcologyMolecular Ecology}, volume = {14}, number = {8}, year = {2005}, pages = {2433-2443}, publisher = {Blackwell Synergy}, keywords = {Reptilia}, author = {Poulakakis, N. and Lymberakis, P. and Efstratios D. Valakos and Pafilis, P. and Zouros, E. and Mylonas, M.} } @article {10619, title = {Phylogenetic relationships and biogeography of Podarcis species from the Balkan Peninsula, by bayesian and maximum likelihood analyses of mitochondrial DNA sequences}, journal = {Molecular Phylogenetics and EvolutionMolecular Phylogenetics and Evolution}, volume = {37}, number = {3}, year = {2005}, pages = {845-857}, abstract = {Wall lizards of the genus Podarcis (Sauria, Lacertidae) comprise 17 currently recognized species in southern Europe, where they are the predominant nonavian reptile group. The taxonomy of Podarcis is complex and unstable. Based on DNA sequence data, the species of Podarcis falls into four main groups that have substantial geographic coherence (Western island group, southwestern group, Italian group, and Balkan Peninsula group). The Balkan Peninsula species are divided into two subgroups: the subgroup of P. taurica (P. taurica, P. milensis, P. gaigeae, and perhaps P. melisellensis), and the subgroup of P. erhardii (P. erhardii and P. peloponnesiaca). In the present study, the question of phylogenetic relationships among the species of Podarcis encountered in the Balkan Peninsula was addressed using partial mtDNA sequences for cytochrome b (cyt b) and 16S rRNA (16S). The data support the monophyly of Podarcis and suggest that there are three phylogenetic clades: the clade A (P. taurica, P. gaigeae, P. milensis, and P. melisellensis); the clade B (P. erhardii and P. peloponnesiaca), and the clade C (P. muralis and P. sicula). By examining intraspecific relationships it was found that extant populations of P. erhardii are paraphyletic. Furthermore, subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, suggesting that their status should be reconsidered. The distinct geographic distribution of the major clades of the phylogenetic tree and its topology suggest a spatial and temporal sequence of phylogenetic separations that coincide with some major paleogeographic separations during the geological history of the Aegean Sea. The results stress the need for a reconsideration of the evolutionary history of Balkan Podarcis species and help overcome difficulties that classical taxonomy has encountered at both the species and subspecies level.}, keywords = {Reptilia}, author = {Poulakakis, N. and Lymberakis, P. and Efstratios D. Valakos and Zouros, E. and Mylonas, M.} } @article {10620, title = {Molecular phylogeny of the Greek legless skink Ophiomorus punctatissimus (Squamata: Scincidae): The impact of the Mid-Aegean trench in its phylogeography}, journal = {Molecular Phylogenetics and EvolutionMolecular Phylogenetics and Evolution}, volume = {47}, number = {1}, year = {2008}, pages = {396}, publisher = {Elsevier}, author = {Poulakakis, N. and Pakaki, V. and Mylonas, M. and Lymberakis, P.} } @article {10621, title = {Molecular phylogeny of the Greek legless skink Ophiomorus punctatissimus (Squamata: Scincidae): the impact of the Mid-Aegean trench in its phylogeography}, journal = {Molecular Phylogenetics and EvolutionMolecular Phylogenetics and Evolution}, volume = {47}, number = {1}, year = {2008}, month = {April}, pages = {396-402}, isbn = {1055-7903}, author = {Poulakakis, N. and Pakaki, V. and Mylonas, M. and Lymberakis, P.} } @article {9674, title = {Digestive performance in five Mediterranean lizard species: effects of temperature and insularity}, journal = {Journal of Comparative Physiology BJournal of Comparative Physiology B}, volume = {177}, number = {1}, year = {2007}, pages = {49-60}, abstract = {Temperature sensitivity of digestive processes has important ramifications for digestive performance in ectothermic vertebrates. We conducted a comparative analysis of temperature effects on digestive processes [gut passage times (GPTs) and apparent digestive efficiencies (ADEs)] in five lacertid lizards occurring in insular (Podarcis erhardii, P. gaigeae), and mainland (P. muralis, P. peloponnesiaca, Lacerta graeca) Mediterranean environments. GPTs were negatively correlated to temperature with mainland taxa having 10-20\% longer GPTs than island taxa. In contrast to previous studies that estimate ADEs using bomb calorimetry, we compare ADEs by analyzing discrete efficiencies for lipids, sugars and proteins at three temperature regimes (20, 25, and 30 degrees C); each of these categories produces different results. ADEs for lipids and sugars showed a monotonic increase with temperature whereas ADEs for proteins decreased with temperature. Island taxa had consistently higher ADEs than their mainland counterparts for lipids and for proteins but not for sugars. They are characterized by superior energy acquisition abilities despite significantly shorter GPTs. Their increased digestive performance relative to the mainland species appears to allow them to maximize energy acquisition in unproductive island environments where food availability is spatially and seasonally clustered.}, keywords = {Reptilia}, author = {Pafilis, P. and Foufopoulos, J. and Poulakakis, N. and Lymberakis, P. and Efstratios D. Valakos} } @article {9242, title = {Isolation and characterization of six polymorphic microsatellite markers in the freshwater turtle Mauremys rivulata (Testudines: Geoemydidae)}, journal = {Molecular Ecology NotesMolecular Ecology Notes}, volume = {5}, year = {2005}, pages = {727-729}, keywords = {Reptilia}, author = {Mantziou, G. and Antoniou, A. and Poulakakis, N. and Goulielmos, G. and Tsigenopoulos, C. 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M., Moritz, C. \& Mable, B. K. (Eds). Sunderland: Sinauer; Taberlet, P., Fumagalli, L., Wust-Saucy, A.G., Cosson, J.F., Comparative phylogeography and postglacial colonization routes in Europe (1998) Molecular Ecology, 7, pp. 453-464; Tajima, F., Simple methods for testing the molecular evolutionary clock hypothesis (1993) Genetics, 135, pp. 599-607; Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tool (1997) Nucleic Acids Research, 24, pp. 4876-4882; Tok, C.V., The taxonomy and ecology of Mauremys caspica rivulata Valenciennes, 1833 (Testudinata: Testudinidae) on Resadiye (Dacta) Peninsula (1999) Turkish Journal of Zoology, 23, pp. 17-21.}, month = {2004///}, pages = {35-45}, abstract = {The genus Mauremys (Chelonia, Bataguridae) is widely distributed throughout Asia, Europe and NW Africa. Three species (Mauremys caspica, Mauremys rivulata and Mauremys leprosa) are discontinuously distributed around the Mediterranean region. Present distributions are much smaller than those documented within the fossil record of Mauremys in the Mediterranean region. All three extant species are identified on the basis of morphology. In the present study we compare partial mitochondrial DNA sequences of cyt-b from 16 populations of Mauremys rivulata from Greece, one from Jordan (M. rivulata), two from Syria (M. caspica) and one from Morocco (M. leprosa). Comparison of cyt-b partial sequences supports the monophyly of the three species considered, as well as their proposed taxonomic status (i.e. separation at the species level). Mauremys leprosa is the most differentiated of the three, M. caspica and M. rivulata being more closely related. Climatic changes during the Pleistocene influenced the distribution of M. rivulata and resulted in a latitudinal oscillation of the populations in a north - south direction in Greece, and consequently in a mixing of their genetic material. This hypothesis is confirmed by the absence of correlation between genetic distances and geographical origin of the specimens studied.}, keywords = {Aegean region, Cytochrome b, Mediterranean, phylogeography, Reptilia}, author = {Mantziou, G. and Poulakakis, N. and Lymberakis, P. and Efstratios D. Valakos and Mylonas, M.} } @article {8934, title = {Two new species of Podarcis (Squamata; Lacertidae) from Greece}, journal = {Systematics and BiodiversitySystematics and Biodiversity}, volume = {6}, number = {03}, year = {2008}, pages = {307}, publisher = {Cambridge Univ Press}, author = {Lymberakis, P. and Poulakakis, N. and Kaliontzopoulou, A. and Efstratios D. Valakos and Mylonas, M.} } @article {8935, title = {Two new species of Podarcis (Squamata; Lacertidae) from Greece}, journal = {Systematics and BiodiversitySystematics and Biodiversity}, volume = {6}, number = {3}, year = {2008}, month = {Sep}, pages = {307-318}, abstract = {Recently, several works have focused on the lacertid lizards of the genus Podarcis, revealing cases of hidden diversity and paraphyly, and offering evidence that suggests the revision of the extant taxonomical arrangements within the genus. Hidden diversity and paraphyly have been shown to exist in the relationships between the Balkan species P. peloponnesiaca and P. erhardii as well. Here we couple a molecular (mtDNA) dataset with a corresponding morphological one, consisting of morphometric and pholidotic characters, to check for concordance between the two. Phylogenetic analyses reinforced previous suggestions for paraphyly of P. erhardii with respect to P. peloponnesiaca. We found the variation of certain pholidotic characters concordant with the relationships inferred from partial mtDNA sequences, whereas morphometric characters were not. The latter is possibly due to greater influence of morphometric characters by environmental factors. To avoid the observed paraphyly we proceed with the description of the populations from Crete and the Islet of Pori, until now designated as P. erhardii, as separate taxa at the species level.}, isbn = {1477-2000}, author = {Lymberakis, P. and Poulakakis, N. and Kaliontzopoulou, A. and Efstratios D. Valakos and Mylonas, M.} } @article {8340, title = {Mitochondrial DNA reveals the genealogical history of the snake-eyed lizards (Ophisops elegans and O. occidentalis)(Sauria: Lacertidae)}, journal = {Molecular Phylogenetics and EvolutionMolecular Phylogenetics and Evolution}, year = {2008}, publisher = {Elsevier}, author = {Kyriazi, P. and Poulakakis, N. and Parmakelis, A. and Crochet, P. A. and Moravec, J. and Rastegar-Pouyani, N. and Tsigenopoulos, C. S. and Magoulas, A. and Mylonas, M. and Lymberakis, P.} }