@article {9373, title = {Phylogenetic relationships of Greek Apis mellifera subspecies based on sequencing of mtDNA segments (COI and ND5)}, journal = {Journal of Apicultural ResearchJournal of Apicultural ResearchJournal of Apicultural Research}, volume = {50}, number = {1}, year = {2011}, note = {ISI Document Delivery No.: 721EDTimes Cited: 1Cited Reference Count: 51}, pages = {42-50}, type = {Article}, abstract = {Honey bee Apis mellifera L. populations from various areas of Albania, Bulgaria, Cyprus, Greece, Italy, Slovenia and Turkey, together with a genetically improved commercial strain exported all over the world were studied using sequencing analysis of two mitochondrial regions, the ND5 and the COI gene segments. The aim of this study was to examine the phylogenetic relationships among these honey bee populations, to investigate the existence of gene flow as a result of migratory beekeeping and commercial breeding, and to find out if these subspecies can be discriminated. Seven and eight different haplotypes were revealed for the COI and ND5 gene segments respectively, while the combined data set consisted of twelve different haplotypes. Among the two DNA segments studied, the highest genetic divergence values were observed in COI. In both genes the highest divergence value was among A. m. ligustica colonies and all others. All the phylogenetic trees constructed by Maximum Parsimony, Neighbour-Joining and Bayesian Inference analyses exhibited exactly the same topology for COI and ND5 separately, as well as for the concatenated data set; A. m. ligustica forms the most distinct clade. Our study presents the first comprehensive sequencing analysis of A. mellifera subspecies occurring in Greece and it is the first time that sequencing data from ND5 mtDNA gene segment have been obtained at the population level.}, keywords = {4TH LINEAGE, ALIGNMENT, Apidae, Apis mellifera, genetic diversity, Greece, HONEY-BEE, Hymenoptera, microsatellite, MITOCHONDRIAL-DNA VARIATION, mtDNA, phylogenetic relationships, populations, sequencing, variability}, isbn = {0021-8839}, author = {Martimianakis, S. and Klossa-Kilia, E. and Bouga, M. and Kilias, G.} } @article {6298, title = {Differences in the morphology of prothoracic and propodeal spiracles in three strains of Apis mellifera: Possible relation to resistance against Acarapis woodi}, journal = {Journal of Apicultural ResearchJournal of Apicultural Research}, volume = {43}, number = {3}, year = {2004}, note = {Cited By (since 1996): 3Export Date: 5 September 2006Source: ScopusReferences: Danka, R.G., Villa, J.D., Evidence of autogrooming as a mechanism of honey bee resistance to tracheal mite infestation (1998) Journal of Apicultural Research, 37, pp. 39-46; Danka, R.G., Villa, J.D., Paravestibular hairs of British Buckfast honey bees do not regulate genetic resistance to Acarapis woodi (1999) Journal of Apicultural Research, 38, pp. 221-223; Danka, R.G., Villa, J.D., Autogrooming by resistant honey bees challenged with individual tracheal mites (2003) Apidologie, 34, pp. 591-596; Delfinado-Baker, M., Baker, E.W., Notes on honey bee mites of the genus Acarapis Hirst (Acari: Tarsonemidae) (1982) International Journal of Acarology, 8, pp. 211-226; Goodman, L., (2003) Form and Function in the Honey Bee, 220p. , IBRA; Cardiff, UK; Lee, D.C., The susceptibility of honey bees of different age to infestations by Acarapis woodi (Renne) (1963) Journal of Insect Pathology, 5, pp. 11-15; Liakos, V., Pasaloglou, M., Katzigiannakis, I., Papadopoulou, K., The tracheal mite disease in Greece (1995) Geotechnical Research Issues, 1, pp. 9-16; Liakos, V., Katzgiannakis, I., Papadakis, I., Mountaki, K., Tracheal acariasis due to Acarapis woodi in bees of Crete (1995) Bulletin of Hellenic Veterinary Medical Society, 46, pp. 35-38; Ruttner, F., (1988) Biogeography and Taxonomy of Honeybees, 267p. , Springer- Verlag; Berlin, Germany; Snodgrass, R.E., (1956) Anatomy of the Honey Bee, 334p. , Cornell University Press Ltd; New York, USA.}, month = {2004///}, pages = {105-113}, abstract = {The prothoracic and propodeal spiracles of three honey bee strains used commercially in Greece (from N Greece, N Italy and Slovenia) were examined with a light microscope in order to detect possible morphological differences between the strains. This is an attempt to explain the apparent resistance against Acarapis woodi shown by the Greek Macedonian (N Greece) strain but not by the others. The propodeal spiracles of honey bees from N Greece and N Italy were examined, in addition, using a scanning electron microscope. Certain dimensions, like the length and the area of aperture in the prothoracic spiracle, were found to be significantly larger in honey bees from N Italy and Slovenia compared with the honey bees from N Greece. The length of aperture was found to be 648 {\`\i}m in the honey bees from N Greece, while it was 13\% larger in the honey bees from N Italy and 10\% larger in the honey bees from Slovenia; the area of the aperture was found to be 103000 {\`\i}m2 in the honey bees from N Greece, while it was 26\% larger in the honey bees from N Italy and 27\% larger in the honey bees from Slovenia. The dimensions of the propodeal spiracle were found to be similar between the strains examined. The fact that the prothoracic spiracle of honey bees from N Greece was significantly smaller compared to other honey bee groups (from N Italy and Slovenia), is discussed as a possible mechanism for resistance developed by this strain against Acarapis woodi.}, keywords = {Acarapis woodi, Apis mellifera, Honey bees, Hymenoptera, morphometrics, Propodeum, Prothorax, resistance, Spiracle}, author = {Hatjina, F. and Gregorc, A. and Papaefthimiou, C. and Pappas, N. and Zacharioudakis, S. and Thrasyvoulou, A. and Theophilidis, G.} } @article {3196, title = {Efficacy of repeated trickle applications of oxalic acid in syrup for varroosis control in Apis mellifera: Influence of meteorological conditions and presence of brood}, journal = {Veterinary ParasitologyVeterinary ParasitologyVeterinary Parasitology}, volume = {148}, number = {2}, year = {2007}, note = {ISI Document Delivery No.: 205VHTimes Cited: 2Cited Reference Count: 16}, month = {Sep}, pages = {174-178}, type = {Article}, abstract = {Oxalic acid field trails for the control of varroosis (Varroa destructor) were carried out in an apiary located on the Mt. Imittos (Attica, Greece). The colonies received four successive applications (approximately one every 16 days) with 4.2\% oxalic acid (OA) and 60\% sugar solution by trickling method with two alternative types of syringes (an automatic self-filling dosing and a single-use) from the broodright to broodless period. The results indicate that the first three applications (from 6th October to 25th November-broodright period) resulted in 65.3\% cumulative mite mortality, while only the last application (after the 26th November-broodless period) resulted in 77.3\% mite mortality. Very low outern temperatures reduce to the minimum the bee movability, which may result into a slower development of the OA efficacy. No poor colony growth or queen loss were observed even if the bee colonies were received the four successive OA applications with the last one taken place at a very low outern temperature (6.2 degrees C). The trickling method using an automatic-filling syringe seems to be a very quick way for applying oxalic acid in large apiaries (approximately 150 hives/h). (c) 2007 Elsevier B.V. All rights reserved.}, keywords = {Apis mellifera, colonies, JACOBSONI, oxalic acid, sucrose, syringe, trickling, Varroa destructor}, isbn = {0304-4017}, author = {Bacandritsos, N. and Papanastasiou, I. and Saitanis, C. and Nanetti, A. and Roinioti, E.} } @conference {2331, title = {POLLINATION ECOLOGY OF Campanula SPECIES ON MT OLYMPOS (GREECE)}, booktitle = {EURECO {\textquoteright}99 - The European Dimension in Ecology - Perspectives \& Challenges for the 21st Century}, year = {1999}, month = {September 18-23}, publisher = {European Ecological Federation}, organization = {European Ecological Federation}, address = {Halkidiki, Greece.}, keywords = {Andrenidae, Apidae, Apis, Apis mellifera, bees, Bombus, Campanula, ecology, Halictidae, Hymenoptera, insects, mellifera, pollination, έντομα, επικονίαση, μέλισσες, οικολογία, Υμενόπτερα}, author = {Blionis, G. J. and Vokou, D.} } @conference {2178, title = {Study of Twelve Morphological Characters in Greek Honeybee Populations Apis mellifera of Thrace and Eptanissa Regions}, booktitle = {ΕΛΛΗΝΙΚΗ ΕΤΑΙΡΕΙΑ ΒΙΟΛΟΓΙΚΩΝ ΕΠΙΣΤΗΜΩΝ 24{\textdegree} ΕΠΙΣΤΗΜΟΝΙΚΟ ΣΥΝΕ∆ΡΙΟ}, year = {2002}, month = {23{\textendash}26 ΜΑΪΟΥ}, publisher = {ΕΛΛΗΝΙΚΗ ΕΤΑΙΡΕΙΑ ΒΙΟΛΟΓΙΚΩΝ ΕΠΙΣΤΗΜΩΝ}, organization = {ΕΛΛΗΝΙΚΗ ΕΤΑΙΡΕΙΑ ΒΙΟΛΟΓΙΚΩΝ ΕΠΙΣΤΗΜΩΝ}, address = {ΕΡΕΤΡΙΑ ΕΥΒΟΙΑΣ}, keywords = {Apis, Apis mellifera, characters, Eptanissa, Honeybee, mellifera, morphological, Thrace, Επτάνησα, Θράκη, μέλισσα, μορφολογικά, χαρακτήρες}, author = {Porichi, A.-E and Harizanis, P.} } @conference {2171, title = {Restricted Analysis of mtDNA in Honeybee Apis mellifera and Phylogenetic Relationships}, booktitle = {ΕΛΛΗΝΙΚΗ ΕΤΑΙΡΕΙΑ ΒΙΟΛΟΓΙΚΩΝ ΕΠΙΣΤΗΜΩΝ 24{\textdegree} ΕΠΙΣΤΗΜΟΝΙΚΟ ΣΥΝΕ∆ΡΙΟ}, year = {2002}, month = {23{\textendash}26 ΜΑΪΟΥ}, publisher = {ΕΛΛΗΝΙΚΗ ΕΤΑΙΡΕΙΑ ΒΙΟΛΟΓΙΚΩΝ ΕΠΙΣΤΗΜΩΝ}, organization = {ΕΛΛΗΝΙΚΗ ΕΤΑΙΡΕΙΑ ΒΙΟΛΟΓΙΚΩΝ ΕΠΙΣΤΗΜΩΝ}, address = {ΕΡΕΤΡΙΑ ΕΥΒΟΙΑΣ}, keywords = {Apis, Apis mellifera, bee, honey, insect, insecta, mellifera, mtDNA, phylogenetic, phylogeny, relationships, έντομο, μέλι, μέλισσα, σχέσεις, φυλογενετική, φυλογενία}, author = {Bouga, M. and Harizanis, P and Kilias, G and Alahiotis, S} }