@article {9373, title = {Phylogenetic relationships of Greek Apis mellifera subspecies based on sequencing of mtDNA segments (COI and ND5)}, journal = {Journal of Apicultural ResearchJournal of Apicultural ResearchJournal of Apicultural Research}, volume = {50}, number = {1}, year = {2011}, note = {ISI Document Delivery No.: 721EDTimes Cited: 1Cited Reference Count: 51}, pages = {42-50}, type = {Article}, abstract = {Honey bee Apis mellifera L. populations from various areas of Albania, Bulgaria, Cyprus, Greece, Italy, Slovenia and Turkey, together with a genetically improved commercial strain exported all over the world were studied using sequencing analysis of two mitochondrial regions, the ND5 and the COI gene segments. The aim of this study was to examine the phylogenetic relationships among these honey bee populations, to investigate the existence of gene flow as a result of migratory beekeeping and commercial breeding, and to find out if these subspecies can be discriminated. Seven and eight different haplotypes were revealed for the COI and ND5 gene segments respectively, while the combined data set consisted of twelve different haplotypes. Among the two DNA segments studied, the highest genetic divergence values were observed in COI. In both genes the highest divergence value was among A. m. ligustica colonies and all others. All the phylogenetic trees constructed by Maximum Parsimony, Neighbour-Joining and Bayesian Inference analyses exhibited exactly the same topology for COI and ND5 separately, as well as for the concatenated data set; A. m. ligustica forms the most distinct clade. Our study presents the first comprehensive sequencing analysis of A. mellifera subspecies occurring in Greece and it is the first time that sequencing data from ND5 mtDNA gene segment have been obtained at the population level.}, keywords = {4TH LINEAGE, ALIGNMENT, Apidae, Apis mellifera, genetic diversity, Greece, HONEY-BEE, Hymenoptera, microsatellite, MITOCHONDRIAL-DNA VARIATION, mtDNA, phylogenetic relationships, populations, sequencing, variability}, isbn = {0021-8839}, author = {Martimianakis, S. and Klossa-Kilia, E. and Bouga, M. and Kilias, G.} } @article {3906, title = {Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR - RFLP analysis of three mtDNA segments}, journal = {ApidologieApidologie}, volume = {36}, number = {3}, year = {2005}, note = {Cited By (since 1996): 2Export Date: 4 September 2006Source: ScopusReferences: Arias, M.C., Sheppard, W.S., Molecular Phylogenetics of Honey Bee Subspecies (Apis mellifera L.) 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Seven, eight and seven restriction enzymes were found to have at least one recognition site at the 16s rDNA, CO I, and ND 5 segments, respectively. Seven different haplotypes were detected and diagnostic patterns enabled us to discriminate A. m. macedonica from the rest of the populations (races). The estimated net nucleotide sequence divergence among the populations examined was found to range from 0.00 to 1.18 with the highest value observed to be between A. m. macedonica and non-A. m. macedonica populations. The trees obtained (by UPGMA and Dollo parsimony methods) revealed that the most distant population was that of A. m. macedonica. {\textcopyright} INRA/DIB-AGIB/ EDP Sciences, 2005.}, keywords = {Apis mellifera, Genetic variability, Greece, Honey bee, mtDNA}, author = {Bouga, M. and Harizanis, P.C. and Kilias, G. and Alahiotis, S.} } @article {3908, title = {Non-lethal DNA sampling of wing tips discriminates subspecies of Apis mellifera occurring in Greece}, journal = {Journal of Apicultural ResearchJournal of Apicultural Research}, volume = {44}, number = {4}, year = {2005}, note = {Export Date: 4 September 2006Source: ScopusReferences: Bouga, M., Tsipi, M., Mavroudis, M., Harizanis, P., Garnery, L., Arnold, G., Tselios, D., Genetic variation in Greek honey bees: Molecular and classical morphometrics approach (2004) Proceedings of the 1st European Conference of Apidology, , Udine, Italy; 39 pp; Bouga, M., Harizanis, P.C., Kilias, G., Alahiotis, S., Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR-RFLP analysis of three mtDNA Segments (2005) Apidologie, 36, pp. 335-344; Cha?line, N., Ratnieks, F.L.W., Raine, N.E., Badcock, N.S., Burke, T., Non-lethal sampling of honey bee, Apis mellifera, DNA using wing tips (2004) Apidologie, 35, pp. 311-318; Harizanis, P., Bouga, M., Genetic structure of the bee from Crete Island (Greece) (2003) XXVIIIth International Apicultural Congress of Apimondia, Final Programme and Book of Abstracts, , Ljubljana, Slovenia; 696 pp; Hatjina, F., Gregorc, A., Papaefthimiou, C., Pappas, N., Zacharioudakis, S., Thrasyvoulou, A., Theophilidis, G., Differences in the morphology of prothoracic and propodeal spiracles in three strains of Apis mellifera: Possible relation to resistance against Acarapis woodi (2004) Journal of Apicultural Research, 43 (3), pp. 105-113; Meusel, M.S., Moritz, R.F.A., Transfer of paternal mitochondrial DNA in fertilization of honeybee (Apis mellifera L) eggs (1993) Current Genetics, 24 (6), pp. 539-543; Ruttner, F., (1988) Biogeography and Taxonomy of Honeybees, , Springer-Verlag; Berlin, Germany; Walsh, P.S., Metzger, D.A., Higuchi, R., Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material (1991) Bio Techniques, 10, pp. 506-513.}, month = {2005///}, pages = {195-196}, keywords = {Apis mellifera, Certification, Greece, Hymenoptera, PCR-RFLP, Queens}, author = {Bouga, M. and Klossa-Kilia, E. and Alahiotis, S. and Kilias, G.} }